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Home >> Antibodies >> Annexin A1 Antibody for WB / ANXA1 Western Blot Antibody

Annexin A1 Antibody for WB / ANXA1 Western Blot Antibody [clone CPTC-ANXA1-1] (V7283)

  Catalog No Formulation Size Price (USD)  
Image V7283-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 559
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V7283-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 259
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V7283SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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V7283IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 559
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Annexin A1 Antibody for WB Multi-Species Lung Tissue WB. Western blot analysis was performed with Annexin A1 Antibody for WB, clone CPTC-ANXA1-1, using lung tissue lysates from human, mouse, rat, hamster, guinea pig and rabbit. Annexin A1 immunoreactive species are detected in the expected molecular weight region in multiple species, with differences in signal intensity and band pattern among samples. This Annexin A1 Antibody demonstrates broad mammalian ANXA1 recognition and supports comparative Western blot studies of Annexin A1 expression across species.
Annexin A1 Antibody for WB Human Tissue and A549 Cell WB. Western blot analysis was performed with Annexin A1 Antibody for WB, clone CPTC-ANXA1-1. Samples include human intestine, human lung, human spleen and A549 cell lysates. Immunoreactive bands are observed in the approximately 33–40 kDa region, encompassing the expected molecular weight of Annexin A1 at approximately 39 kDa. This Annexin A1 Antibody demonstrates ANXA1 detection across multiple human tissues and cells and supports Western blot studies of Annexin A1 expression.
Annexin A1 Antibody for WB Human A549 Lung Cancer Cell WB. Western blot analysis of human A549 lung cancer cell lysate was performed with Annexin A1 Antibody for WB, clone CPTC-ANXA1-1. A prominent band is detected at approximately 39 kDa, consistent with the expected molecular weight of Annexin A1. This ANXA1 Western Blot Antibody demonstrates detection of endogenous Annexin A1 in human A549 lung cancer cells and supports studies of ANXA1 expression in cancer and inflammatory signaling.
Annexin A1 Antibody Human Colon Carcinoma IHC. Immunohistochemistry staining of FFPE human colon carcinoma tissue was performed with Annexin A1 Antibody, clone CPTC-ANXA1-1. Strong positive staining is observed predominantly in stromal and inflammatory cells surrounding the colonic glands, while the glandular epithelial cells show comparatively little staining. This Annexin A1 Antibody demonstrates ANXA1 expression in the human colon carcinoma microenvironment and complements its validated Western blot performance for studies of inflammatory signaling and cancer biology.
Annexin A1 Antibody Human Placenta IHC. Immunohistochemistry staining of FFPE human placenta tissue was performed with Annexin A1 Antibody, clone CPTC-ANXA1-1. Positive staining is observed prominently along placental villous structures, including trophoblast-associated regions. This Annexin A1 Antibody demonstrates ANXA1 expression in human placenta and complements its validated Western blot performance for studies of Annexin A1 expression and inflammatory signaling.
Annexin A1 Antibody CPTC-ANXA1-1 Purity SDS-PAGE. SDS-PAGE analysis of purified Annexin A1 Antibody, clone CPTC-ANXA1-1, was performed under non-reducing (NR) and reducing (R) conditions with 2 ug of antibody loaded per lane. The non-reduced sample shows the intact antibody, while reduction produces bands corresponding to the immunoglobulin heavy and light chains at approximately 50 and 25 kDa, respectively. This analysis confirms the purity and structural integrity of the mouse monoclonal ANXA1 antibody preparation.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat, Hamster, Rabbit
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name CPTC-ANXA1-1
Purity Protein G affinity chromatography
UniProt P04083
Localization Nuclear and Cytoplasmic
Applications Western Blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Annexin A1 Antibody for WB / ANXA1 Western Blot Antibody is available for research use only.
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Description

Annexin A1 Antibody for WB / ANXA1 Western Blot Antibody is a mouse monoclonal antibody, clone CPTC-ANXA1-1, targeting Annexin A1, a calcium-dependent phospholipid-binding protein encoded by the ANXA1 gene. Annexin A1 belongs to the annexin family and has a predicted molecular weight of approximately 39 kDa. The protein participates in membrane-associated processes, inflammatory regulation, cell signaling and responses to cellular stress, making ANXA1 relevant to research spanning immunology, inflammation and cancer biology.

Annexin A1 was historically characterized as lipocortin I because of its association with glucocorticoid-mediated anti-inflammatory effects. ANXA1 can regulate leukocyte activity and inflammatory responses and has been studied in processes involving neutrophil migration, macrophage function and resolution of inflammation. Its functions are influenced by cellular localization, phosphorylation, proteolytic processing and interactions with membranes and signaling proteins. An Annexin A1 Antibody for WB can support analysis of ANXA1 abundance and molecular weight patterns across different tissues, cell types and experimental conditions.

ANXA1 is also extensively investigated in cancer research. Altered Annexin A1 expression has been reported in multiple malignancies, although its relationship with tumor biology can vary according to tissue and cellular context. Research has connected ANXA1 with cellular proliferation, migration, invasion, differentiation and interactions between tumor cells and the surrounding microenvironment. These diverse functions make analysis of Annexin A1 protein expression relevant to studies of both normal cellular physiology and disease-associated signaling.

Western blot analysis commonly detects Annexin A1 near its expected molecular weight of approximately 39 kDa. Additional immunoreactive species in the neighboring molecular weight range may also be observed depending on sample type and experimental conditions. Annexin A1 is known to undergo post-translational modification and proteolytic processing, particularly involving its N-terminal region, which can contribute to molecular heterogeneity. Clone CPTC-ANXA1-1 has demonstrated Western blot detection across human cells and tissues as well as samples from multiple mammalian species, providing useful data for comparative ANXA1 expression studies.

NSJ Bioreagents offers mouse monoclonal Annexin A1 Antibody clone CPTC-ANXA1-1 for Western blot-based investigation of ANXA1 expression. An ANXA1 Western Blot Antibody can support research into inflammatory regulation, immune signaling, membrane biology, cellular stress and cancer-associated changes in Annexin A1 expression.

Researchers studying Annexin A1 expression and function can explore our Protein Microarray Validated Annexin A1 Antibody page for additional reagents targeting ANXA1 in inflammatory signaling, immune regulation and cancer biology.

Application Notes

Optimal dilution of the Annexin A1 Antibody for WB / ANXA1 Western Blot Antibody to be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant full-length human protein was used as the immunogen for the Annexin A1 antibody.

Storage

Store the Annexin A1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Annexin A1 antibody, ANXA1 antibody, ANXA1 Western Blot antibody, Annexin A1 Western Blot antibody, Annexin A1 Antibody for WB, Lipocortin I antibody, Lipocortin 1 antibody, Annexin I antibody, Calpactin II antibody

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