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Home >> Antibodies >> Annexin A1 Antibody

Annexin A1 Antibody [clone ANXA1/6452R] (V9510)

  Catalog No Formulation Size Price (USD)  
Image V9510-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
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V9510-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
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V9510SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated Recrabbitmono
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Annexin A1 Antibody Human Tonsil Immune Cell IHC. Immunohistochemistry staining of FFPE human tonsil tissue with Annexin A1 Antibody (clone ANXA1/6452R). Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 min followed by cooling before staining. Strong Annexin A1 staining is observed in immune cell populations within the tonsil tissue.
IHC staining of FFPE human tonsil tissue with Annexin A1 antibody (clone ANXA1/6452R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human A549 cell lysate using Annexin A1 antibody (clone ANXA1/6452R). Predicted molecular weight ~36 kDa.
SDS-PAGE analysis of purified, BSA-free Annexin A1 antibody (clone ANXA1/6452R) as confirmation of integrity and purity.
Annexin A1 Antibody Human, Mouse, Rat, Hamster, Guinea Pig and Rabbit Lung WB. Western blot analysis of human, mouse, rat, hamster, guinea pig and rabbit lung tissue lysates using Annexin A1 Antibody (clone rANXA1/9145). Annexin A1 is detected at approximately 37-40 kDa in human, hamster, guinea pig and rabbit lung tissue, with little to no detectable signal observed in the mouse and rat samples under these conditions.
Annexin A1 Antibody Human A549 Lung Cancer Cell and Tissue WB. Western blot analysis of human intestine, human lung and A549 lung cancer cell lysates using Annexin A1 Antibody (clone ANXA1/6452R). Annexin A1 is detected at approximately 37-40 kDa across the samples tested, demonstrating recognition of ANXA1 in human tissues and A549 lung cancer cells.
Availability 1-3 business days
Species Reactivity Human, Rabbit, Hamster, Guinea pig
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name ANXA1/6452R
Purity Protein A/G affinity
UniProt P04083
Localization Nucleus, Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Western Blot : 2-4ug/ml
Limitations This Annexin A1 antibody is available for research use only.
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Description

The ANXA1 gene belongs to the annexin family, and contains 4 annexin repeats. A pair of annexin repeats may form one binding site for calcium and a phospholipid. ANXA1 promotes membrane fusion and is involved in exocytosis. The gene for ANXA1 is upregulated in hairy cell leukemia (HCL), and its protein expression is specific for HCL. Detection of ANXA1 provides a simple, highly sensitive and specific assay for diagnosing HCL. Annexin A1 has also been found to be protective against DNA damage induced by heat in breast cancer cells, suggesting it is involved in tumor suppressive and protective activities, and also is associated with treatment resistance.

Researchers studying Annexin A1 expression and function can explore our Protein Microarray Validated Annexin A1 Antibody page for additional reagents targeting ANXA1 in inflammatory signaling, immune regulation and cancer biology.

Application Notes

Optimal dilution of the Annexin A1 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human Annexin A1 protein was used as the immunogen for the Annexin A1 antibody.

Storage

Aliquot the Annexin A1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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