Annexin A1 Antibody / Lipocortin I Antibody [clone ANXA1/3566] (V7838)

Annexin A1 Antibody Human HeLa Cell IF. Immunofluorescence staining of PFA-fixed human HeLa cells was performed with Annexin A1 Antibody, clone ANXA1/3566 (green), with Reddot nuclear counterstain (red). Annexin A1 staining is observed predominantly throughout the cytoplasmic compartment, with a punctate intracellular distribution. This Annexin A1 Antibody demonstrates ANXA1 localization in human HeLa cells and supports studies of membrane-associated processes, inflammatory signaling and cancer biology.

Annexin A1 Antibody Human Lymph Node IHC. Immunohistochemistry staining of FFPE human lymph node tissue was performed with Annexin A1 Antibody, clone ANXA1/3566. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10–20 min followed by cooling. Strong positive staining is observed in numerous cells throughout the lymph node tissue. This Annexin A1 Antibody demonstrates ANXA1 expression in human lymphoid tissue and supports studies of inflammatory regulation and immune signaling.

Annexin A1 Antibody Human Lymph Node Immune Cell IHC. Immunohistochemistry staining of FFPE human lymph node tissue was performed with Annexin A1 Antibody, clone ANXA1/3566. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10–20 min followed by cooling. Strong positive staining is observed in numerous immune cells throughout the lymphoid tissue. This Annexin A1 Antibody demonstrates ANXA1 expression in human lymph node and supports studies of inflammatory regulation and immune signaling.

Annexin A1 Antibody Human Colon Carcinoma IHC. Immunohistochemistry staining of FFPE human colon carcinoma tissue was performed with Annexin A1 Antibody, clone ANXA1/3566. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10–20 min followed by cooling. Strong positive staining is observed predominantly in stromal cells surrounding the malignant colonic glands, while the epithelial tumor cells show comparatively little staining. This Annexin A1 Antibody demonstrates ANXA1 expression within the human colon carcinoma microenvironment and supports studies of inflammatory signaling and cancer biology.

Annexin A1 Antibody Human Prostate Carcinoma IHC. Immunohistochemistry staining of FFPE human prostate carcinoma tissue was performed with Annexin A1 Antibody, clone ANXA1/3566. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10–20 min followed by cooling. Strong positive staining is observed in the malignant glandular epithelial cells. This Annexin A1 Antibody demonstrates ANXA1 expression in human prostate carcinoma and supports studies of Annexin A1 in epithelial tumor biology and cancer-associated signaling.

Western blot testing of human A549 cell lysate with Annexin A1 antibody (clone ANXA1/3566). Predicted molecular weight ~38 kDa.

Annexin A1 Antibody Human HeLa Cell FACS. Flow cytometry analysis of PFA-fixed human HeLa cells was performed with Annexin A1 Antibody, clone ANXA1/3566. The blue histogram represents cells stained with Annexin A1 Antibody, while the red histogram represents the isotype control. A clear fluorescence shift is observed with the Annexin A1 Antibody compared with the isotype control, demonstrating detection of ANXA1 in human HeLa cells and supporting studies of Annexin A1 expression and immune-related signaling.

Annexin A1 Antibody Protein Microarray Specificity Validation. Protein Microarray Validated Annexin A1 Antibody, clone ANXA1/3566, was analyzed using a HuProt microarray containing more than 19,000 full-length human proteins. ANXA1 produced the highest-ranked signal with a Z-score of 113.3 and an S-score of 96.14, demonstrating strong preferential recognition of the intended target. The Z-score represents signal strength in standard deviations above the mean signal across the array, while the S-score represents the difference between the target Z-score and the next-ranked protein and therefore reflects relative target specificity. This Annexin A1 Antibody demonstrates a high degree of specificity for ANXA1 across the human protein microarray.

SDS-PAGE analysis of purified, BSA-free Annexin A1 antibody (clone ANXA1/3566) as confirmation of integrity and purity.

Western blot testing of human A549 cell lysate with Annexin A1 antibody (clone ANXA1/3566). Predicted molecular weight ~38 kDa.