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Home >> Antibodies >> Annexin A1 Antibody

Annexin A1 Antibody [clone 6E4/3] (V3741)

  Catalog No Formulation Size Price (USD)  
Image V3741-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V3741-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V3741SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V3741IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC testing of FFPE human bladder carinoma with Annexin A1 antibody (clone 6E4/3). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free Annexin A1 antibody (clone 6E4/3) as confirmation of integrity and purity.
Flow testing of fixed and permeabilized human HeLa cells with Annexin A1 antibody (clone 6E4/3). Red=isotype control, Blue= Annexin A1 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name 6E4/3
Purity Protein G affinity chromatography
UniProt P04083
Localization Nuclear and Cytoplasmic
Applications Flow cytometry : 1-2ug/10^6 cells
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Annexin A1 antibody is available for research use only.
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Description

The ANXA1 gene belongs to the annexin family, and contains 4 annexin repeats. A pair of annexin repeats may form one binding site for calcium and a phospholipid. ANXA1 promotes membrane fusion and is involved in exocytosis. The gene for ANXA1 is upregulated in hairy cell leukemia (HCL), and its protein expression is specific for HCL. Detection of ANXA1 provides a simple, highly sensitive and specific assay for diagnosing HCL. Annexin A1 has also been found to be protective against DNA damage induced by heat in breast cancer cells, suggesting it is involved in tumor suppressive and protective activities, and also is associated with treatment resistance.

Application Notes

Optimal dilution of the Annexin A1 antibody to be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human full-length protein was used as the immunogen for the Annexin A1 antibody.

Storage

Store the Annexin A1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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