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Home >> Antibodies >> ANKH Antibody

ANKH Antibody (F55041)

  Catalog No Formulation Size Price (USD)  
Image F55041-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 369
Image
F55041-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 155
Western blot testing of mouse cerebellum tissue lysate with ANKH antibody. Predicted molecular weight ~54 kDa.
Western blot testing of human U-2 OS cell lysate with ANKH antibody. Predicted molecular weight ~54 kDa.
IHC testing of FFPE human brain tissue with ANKH antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human K562 cells with ANKH antibody; Blue=isotype control, Green= ANKH antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
UniProt Q9HCJ1
Applications Flow cytometry : 1:10-1:50 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:10-1:50
Western blot : 1:500-1:1000
Limitations This ANKH antibody is available for research use only.
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Description

ANKH is a multipass transmembrane protein that is expressed in joints and other tissues and controls pyrophosphate levels in cultured cells. Progressive ankylosis-mediated control of pyrophosphate levels has been suggested as a possible mechanism regulating tissue calcification and susceptibility to arthritis in higher animals.

Application Notes

The stated application concentrations are suggested starting points. Titration of the ANKH antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 464-492 from the human protein was used as the immunogen for the ANKH antibody.

Storage

Aliquot the ANKH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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