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Home >> Antibodies >> Angiotensinogen Antibody [Discontinued]

Angiotensinogen Antibody [Discontinued] (R30727)

  Catalog No Formulation Size Price (USD)  
Image R30727 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 275
Western blot testing of Angiotensinogen antibody and Lane 1: mouse liver; 2: rat liver; 3: rat heart
Availability Discontinued
Species Reactivity Mouse, Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P11859
Applications Western blot : 0.5-1ug/ml
Limitations This Angiotensinogen antibody is available for research use only.
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Description

AGT (Angiotensinogen), also called SERPINA8, is an alpha-2-globulin that is produced constitutively and released into the circulation mainly by the liver. ATG is a member of the serpin family, although it is not known to inhibit other enzymes, unlike most serpins. The AGT gene is mapped on 1q42.2, and the human gene contains 5 exons. The expression of AGT and enzymes required for its conversion to angiotensin II in human adipose tissue. Western blot analysis showed a distinct band of expected size of the angiotensinogen protein(61 kD) in isolated adipocytes. The more pronounced AGT increase in TT subjects resulted in similar plasma renin activity at a lower plasma active renin concentration, with a higher plasma renin activity/active renin ratio.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Angiotensinogen antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the N-terminus of mouse Angiotensinogen (DRVYIHPFHLLY) was used as the immunogen for this Angiotensinogen antibody (100% rat homology).

Storage

After reconstitution, the Angiotensinogen antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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