Angiopoietin Receptor Antibody | TIE2 [clone TEK/9412] (V6010)

Immunohistochemistry analysis of Angiopoietin Receptor / TIE2 antibody in human testis tissue (clone TEK/9412). FFPE human testis sections demonstrate HRP-DAB brown cytoplasmic and membranous staining within cells of the seminiferous tubules, with additional signal highlighting vascular endothelial cells in the interstitial spaces. The staining pattern is consistent with TEK receptor tyrosine kinase localization, showing membranous accentuation and diffuse cytoplasmic positivity in endothelial-associated structures. The inset shows PBS used in place of primary antibody as a secondary antibody negative control, confirming absence of specific brown chromogenic signal. Heat induced epitope retrieval was performed in 10 mM Tris with 1 mM EDTA, pH 9.0, by heating tissue sections at 95oC for 45 minutes followed by cooling at room temperature for 20 minutes prior to antibody incubation.

Immunohistochemistry analysis of Angiopoietin Receptor / TIE2 antibody in human renal cell carcinoma tissue (clone TEK/9412). FFPE human renal cell carcinoma sections show HRP-DAB brown membranous staining outlining tumor-associated vascular endothelial cells, with focal cytoplasmic signal in adjacent epithelial structures. The staining pattern highlights microvascular components within the tumor stroma, consistent with TEK receptor tyrosine kinase localization. Tumor epithelial cells display weaker and more variable staining compared to endothelial elements. The inset shows PBS used in place of primary antibody as a secondary antibody negative control, confirming absence of specific brown chromogenic signal. Heat induced epitope retrieval was performed in 10 mM Tris with 1 mM EDTA, pH 9.0, by heating tissue sections at 95oC for 45 minutes followed by cooling at room temperature for 20 minutes prior to antibody incubation.

Western blot analysis of Angiopoietin Receptor / TIE2 antibody in human spleen lysate (clone TEK/9412). A distinct immunoreactive band is detected at approximately 140-145 kDa, consistent with the predicted molecular weight of TEK receptor tyrosine kinase. The band appears as a single prominent species without significant non-specific background under the conditions tested. TEK is known to undergo glycosylation, which may contribute to slight migration variability on SDS-PAGE. Molecular weight markers are shown at left.