- Tel: 858.663.9055
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Angiopoietin 2 Antibody (R30172)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
 |
R30172 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 429 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Antigen affinity purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity |
| Buffer | Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal |
| UniProt | O15123 |
| Applications | Western blot : 0.5-1ug/ml |
| Limitations | This Angiopoietin 2 antibody is available for research use only. |
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Angiopoietin 2 and -1 are involved in the development of the endothelium by regulating tyrosine phosphorylation of the membrane receptor Tie 2. Angiopoietin 2 is only 60% homologous with -1. It is a naturally occurring antagonist of ANGPT1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation. ANGPT1 binding to Tie 2 causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.
The stated application concentrations are suggested starting amounts. Titration of the Angiopoietin 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Amino acids 454-468 (NLNGMYYPQRQNTNK-human) were used as the immunogen for this Angiopoietin 2 antibody.
After reconstitution, the Angiopoietin 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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