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Home >> Antibodies >> Angiopoietin 2 Antibody / ANG2 / ANGPT2

Angiopoietin 2 Antibody / ANG2 / ANGPT2 (F54532)

  Catalog No Formulation Size Price (USD)  
Image F54532-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 369
Image
F54532-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 149
IHC testing of FFPE human uterus tissue with Angiopoietin 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human liver lysate with Angiopoietin 2 antibody. Predicted molecular weight ~57 kDa.
Western blot testing of mouse kidney lysate with Angiopoietin 2 antibody. Predicted molecular weight ~57 kDa.
Flow cytometry testing of fixed and permeabilized human A549 cells with Angiopoietin 2 antibody; Blue=isotype control, Green= Angiopoietin 2 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt O15123
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:25
Flow cytometry : 1:25 (1x10e6 cells)
Limitations This Angiopoietin 2 antibody is available for research use only.
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Description

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Angiopoietin 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 404-432 from the human protein was used as the immunogen for the Angiopoietin 2 antibody.

Storage

Aliquot the Angiopoietin 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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