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Home >> Antibodies >> Angiopoietin 1 Antibody [Discontinued, view alternative antibodies]

Angiopoietin 1 Antibody [Discontinued, view alternative antibodies] (R30398)

  Catalog No Formulation Size Price (USD)  
Image R30398 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of Angiopoietin 1 antibody and Lane 1: rat heart; 2: rat brain; 3: rat kidney; 4: human SMMC-7721 cell lysate. Observed size 57~75KD depending on glycosylation level
IHC-P: Angiopoietin 1 antibody testing of human lung cancer tissue
Availability Discontinued
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt Q15389
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
ELISA : 0.1-0.5ug/ml (human protein tested); request BSA free format for coating
Limitations This Angiopoietin 1 antibody is available for research use only.
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Description

Angiopoietin 1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Angiopoietin-1 seems to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Endothelial Tie2/Tek ligands angiopoietin-1(ANGPT1) and angiopoietin-2(ANGPT2): regional localization of the human genes to 8q22.3-q23 and 8p23.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Angiopoietin 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids 225-239 (RQTYIIQELEKQLNR-human) were used as the immunogen for this Angiopoietin 1 antibody.

Storage

After reconstitution, the Angiopoietin 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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