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Home >> Antibodies >> Anaplastic Lymphoma Kinase Antibody / ALK

Anaplastic Lymphoma Kinase Antibody / ALK [clone ALK/1503] (V5625)

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  Catalog No Formulation Size Price (USD)  
Image V5625-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V5625-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V5625SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Western blot testing (A) recombinant protein and (B) human HepG2 cell lysate using Anaplastic Lymphoma Kinase antibody (clone ALK/1503). Predicted molecular weight ~180 kDa but may be observed at higher molecular weights due to glycosylation. Caspase 3 induced cleavage results in an ~60 kDa intracellular ALK fragment.
Western blot testing (A) recombinant protein and (B) human HepG2 cell lysate using Anaplastic Lymphoma Kinase antibody (clone ALK/1503). Predicted molecular weight ~180 kDa but may be observed at higher molecular weights due to glycosylation. Caspase 3 induced cleavage results in an ~60 kDa intracellular ALK fragment.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name ALK/1503
Purity Protein A/G affinity
UniProt Q9UM73
Localization Cell membrane
Applications Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-3ug/ml
Western blot : 2-4ug/ml
Limitations This Anaplastic Lymphoma Kinase antibody is available for research use only.
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Description

The wild-type anaplastic lymphoma kinase (ALK) protein is a 200kDa transmembrane receptor tyrosine kinase. Its expression is restricted to a few scattered cells in the nervous system (some glial cells and neurons, and a few endothelial cells and pericytes. The hybrid gene, NPM-ALK, created by the t(2;5)(p23;q35) chromosomal translocation encodes part of the nucleolar phosphoprotein, nucleophosmin (NPM), joined to the entire cytoplasmic portion of the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. As a consequence, the ALK gene comes under the control of the NPM promoter, which induces a permanent and ubiquitous transcription of the NPM-ALK hybrid gene, resulting in the production of a 80kDa NPM-ALK chimeric protein. This translocation is found in anaplastic large cell lymphomas (ALCL). Reportedly, expression of ALK indicates a better prognosis. Approximately 5%-10% of non-small cell lung carcinomas also express ALK protein producing a cytoplasmic staining pattern. This MAb also reacts with blood vessels that serves as an internal positive control.

Application Notes

Optimal dilution of the Anaplastic Lymphoma Kinase antibody should be determined by the researcher.

Immunogen

Recombinant human Anaplastic Lymphoma Kinase protein fragment (amino acids 1360-1460) was used as the immunogen for the Anaplastic Lymphoma Kinase antibody.

Storage

Aliquot the Anaplastic Lymphoma Kinase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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