APP Antibody / Amyloid Precursor Protein Antibody (R31517)

APP Antibody in Human Cell Lines and Rodent Brain WB. Western blot testing of human HeLa, U-87 MG, T-47D, A549 and U-2 OS cell lysates, rat brain lysate, and mouse brain lysate with APP Antibody detects a prominent species at approximately 110-120 kDa across all samples, with an additional band near 50-55 kDa. The higher molecular weight signal is consistent with glycosylated amyloid precursor protein, while the lower band may represent an APP-derived processing product. This Amyloid Precursor Protein Antibody therefore recognizes multiple APP-associated species under the tested conditions.

APP Antibody in Human A431 Cells IF. Immunofluorescence staining of FFPE human A431 cells with APP Antibody at 2 ug/ml demonstrates green cytoplasmic staining with a granular distribution and areas of stronger perinuclear signal. DAPI counterstaining identifies nuclei in blue, which remain largely devoid of green fluorescence. The observed intracellular pattern is consistent with the membrane-trafficking and vesicular distribution associated with amyloid precursor protein.

APP Antibody in Human Glioma Tissue IHC. Immunohistochemistry staining of FFPE human glioma tissue with APP Antibody at 1 ug/ml demonstrates heterogeneous brown cytoplasmic staining within tumor cells, with areas of more prominent granular positivity. Sections underwent HIER in 10 mM citrate buffer, pH 6, for 10-20 min followed by cooling before testing. The Amyloid Precursor Protein Antibody highlights APP-associated staining against the hematoxylin-counterstained tumor tissue.

APP Antibody in Mouse Brain Tissue IHC. Immunohistochemistry staining of FFPE mouse brain with APP Antibody at 1 ug/ml demonstrates widespread brown staining with prominent cytoplasmic and neuropil-associated signal in the examined brain region. HIER was performed in 10 mM citrate buffer, pH 6, for 10-20 min followed by cooling. The Amyloid Precursor Protein Antibody produces a distinct regional staining pattern while nuclei remain predominantly hematoxylin counterstained.

APP Antibody in Rat Brain Tissue IHC. Immunohistochemistry staining of FFPE rat brain with APP Antibody at 1 ug/ml shows heterogeneous brown staining concentrated in cellular and neuropil-rich regions, with scattered cells displaying stronger cytoplasmic positivity. Sections underwent HIER in 10 mM citrate buffer, pH 6, for 10-20 min followed by cooling. The distribution demonstrates regionally variable recognition by the Amyloid Precursor Protein Antibody in rat brain tissue.

APP Antibody in Human Renal Cancer Tissue IHC. Immunohistochemistry staining of FFPE human renal cancer tissue with APP Antibody at 1 ug/ml demonstrates heterogeneous brown cytoplasmic staining within tumor epithelial structures, with stronger signal in subsets of cells. HIER was performed in 10 mM citrate buffer, pH 6, for 10-20 min followed by cooling. The Amyloid Precursor Protein Antibody produces localized tumor-cell staining against the hematoxylin-counterstained tissue.

APP Antibody in Human Tonsil Tissue IHC. Immunohistochemistry staining of FFPE human tonsil with APP Antibody at 1 ug/ml demonstrates relatively sparse, heterogeneous brown staining within the lymphoid tissue, including scattered positive cells and focal areas of increased signal. Sections underwent HIER in 10 mM citrate buffer, pH 6, for 10-20 min followed by cooling. The Amyloid Precursor Protein Antibody highlights a restricted cellular distribution against the predominantly hematoxylin-counterstained tonsillar tissue.