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Home >> Antibodies >> AMSH-like protease Antibody / STAMBPL1

AMSH-like protease Antibody / STAMBPL1 (F54819)

  Catalog No Formulation Size Price (USD)  
Image F54819-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 359
Image
F54819-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 149
Western blot testing of human HeLa cell lysate with AMSH-like protease antibody. Predicted molecular weight ~50 kDa.
Western blot testing of mouse testis tissue lysate with AMSH-like protease antibody. Predicted molecular weight ~50 kDa.
IHC testing of FFPE human spleen tissue with AMSH-like protease antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human CCRF-CEM cells with AMSH-like protease antibody; Blue=isotype control, Green= AMSH-like protease antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q96FJ0
Localization Cytoplasmic
Applications Flow cytometry : 1:10-1:50 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:50-1:100
Western blot : 1:500-1:1000
Limitations This AMSH-like protease antibody is available for research use only.
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Description

STAMBPL1 is zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains.

Application Notes

The stated application concentrations are suggested starting points. Titration of the AMSH-like protease antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 135-163 from the human protein was used as the immunogen for the AMSH-like protease antibody.

Storage

Aliquot the AMSH-like protease antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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