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Home >> Antibodies >> AMPK gamma 2 Antibody / PRKAG2

AMPK gamma 2 Antibody / PRKAG2 (RQ7196)

  Catalog No Formulation Size Price (USD)  
Image RQ7196 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 419
Western blot testing of 1) human 293T, 2) human HeLa, 3) human placenta, 4) human U-87 MG, 5) human A431, 6) rat heart and 7) mouse NIH 3T3 cell lysate with AMPK gamma 2 antibody. Predicted molecular weight ~63 kDa.
Flow cytometry testing of human PC-3 cells with AMPK gamma 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AMPK gamma 2 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9UGJ0
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This AMPK gamma 2 antibody is available for research use only.
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Description

5'-AMP-activated protein kinase subunit gamma-2 is an enzyme that in humans is encoded by the PRKAG2 gene. AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Application Notes

Optimal dilution of the AMPK gamma 2 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids K24-H530) was used as the immunogen for the AMPK gamma 2 antibody.

Storage

After reconstitution, the AMPK gamma 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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