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Home >> Antibodies >> AMPK Antibody / PRKAA1

AMPK Antibody / PRKAA1 (F40150)

  Catalog No Formulation Size Price (USD)  
Image F40150-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 329
Image
F40150-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
Western blot analysis of AMPK antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKAA1 gene (2).
IHC analysis of FFPE human breast carcinoma tissue stained with AMPK antibody
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt Q13131
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Limitations This AMPK antibody is available for research use only.
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Description

PRKAA1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.

Application Notes

Titration of the AMPK antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 474-502 from human PRKAA1 was used as the immunogen for this AMPK antibody.

Storage

Aliquot the AMPK antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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