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Home >> Antibodies >> AMPK alpha 1 Antibody / PRKAA1

AMPK alpha 1 Antibody / PRKAA1 (RQ4362)

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  Catalog No Formulation Size Price (USD)  
Image RQ4362 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human A549 cells with AMPK alpha 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human A549 cells with AMPK alpha 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human SiHa cells with AMPK alpha 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AMPK alpha 1 antibody.
Western blot testing of human 1) PANC-1 and 2) Jurkat cell lysate with AMPK alpha 1 antibody at 0.5ug/ml. Predicted molecular weight ~63 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q13131
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Direct ELISA : 0.1-0.5ug/ml
Immunofluorescence (FFPE) : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This AMPK alpha 1 antibody is available for research use only.
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Description

5'-AMP-activated protein kinase catalytic subunit alpha-1 is an enzyme that in humans is encoded by the PRKAA1 gene. The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.

Application Notes

Optimal dilution of the AMPK alpha 1 antibody should be determined by the researcher.

Immunogen

A recombinant human protein corresponding to amino acids F377-R446 was used as the immunogen for the AMPK alpha 1 antibody.

Storage

After reconstitution, the AMPK alpha 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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