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Home >> Antibodies >> Aminopeptidase-N Antibody / CD13

Aminopeptidase-N Antibody / CD13 [clone APN/6998] (V4024)

  Catalog No Formulation Size Price (USD)  
Image V4024-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 499
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V4024-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 219
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V4024SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 499
IHC staining of FFPE human tonsil with CD13 antibody (clone APN/6998). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate tissue with CD13 antibody (clone APN/6998). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human kidney with CD13 antibody (clone APN/6998). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human kidney tissue lysate using CD13 antibody (clone APN/6998). Expected molecular weight: 110-150 kDa depending on glycosylation level.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name APN/6998
Purity Protein A/G affinity
UniProt P15144
Applications ELISA : order antibody without BSA for coating
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Aminopeptidase-N antibody is available for research use only.
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Description

Recognizes an integral membrane glycoprotein of 150kDa, identified as CD13 (also known as aminopeptidase-N). The antibody recognizes an extracellular epitope. The CD13 antigen is present on most cells of myeloid origin including granulocytes, monocytes, mast cells, and GM-progenitor cells. It is also expressed by the majority of AML, CML in myeloid blast crisis, and in a smaller fraction of lymphoid leukemias. CD13 is absent from normal lymphocytes, platelets and erythrocytes. CD13 is also present on fibroblasts; endothelial cells, epithelial cells from renal proximal tubules and intestinal brush border, bone marrow stromal cells, osteoclasts, and cells lining bile duct canaliculi. CD13 is identical to aminopeptidase N (APN), a prominent membrane-bound metalloprotease present on the surface of intestinal brush border and renal tubules. CD13 plays a role in metabolism of biologically active peptides, in phagocytosis, and in bactericidal / tumoricidal activities. It also serves as a receptor for human coronaviruses (HCV). The lineage-restricted pattern of expression of CD13 within the hemopoietic compartment suggests that it may be important in myeloid cell differentiation.

Application Notes

Optimal dilution of the Aminopeptidase-N antibody should be determined by the researcher.

Immunogen

A portion of amino acids 50-250 from the human protein was used as the immunogen for the Aminopeptidase-N antibody.

Storage

Aliquot the Aminopeptidase-N antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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