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Home >> Antibodies >> AMACR Antibody

AMACR Antibody (V2506)

  Catalog No Formulation Size Price (USD)  
Image V2506-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V2506-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V2506SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V2506IHC-7ML Prediluted in 1X PBS, 0.05% sodium azide; *For IHC use only* 7 ml 519
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Formalin-fixed, paraffin-embedded human prostate carcinoma stained with AMACR antibody.
Formalin-fixed, paraffin-embedded human lung carcinoma stained with AMACR antibody.
Western blot testing of human kidney lysate with AMACR antibody. Predicted molecular weight ~43 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Protein A affinity chromatography
UniProt Q9UHK6
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1:50-1:100 for 30-60 min at RT
Western blot : 1:50-1:100
Limitations This AMACR antibody is available for research use only.
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Description

This antibody recognizes a protein of 44kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and beta-oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.

Application Notes

Optimal dilution of the AMACR antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A synthetic peptide from the human protein was used as the immunogen for the AMACR antibody.

Storage

Store the AMACR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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