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Home >> Antibodies >> AMACR Antibody (Prostate Cancer Marker)

AMACR Antibody (Prostate Cancer Marker) [clone AMACR/1723] (V3237)

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  Catalog No Formulation Size Price (USD)  
Image V3237-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3237-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3237SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Western blot testing of human PC3 cell lysate with AMACR antibody (clone AMACR/1723). Predicted molecular weight ~43 kDa.
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Western blot testing of human PC3 cell lysate with AMACR antibody. Predicted molecular weight ~43 kDa.
Western blot testing of human kidney lysate with AMACR antibody. Predicted molecular weight ~43 kDa.
Western blot testing of human liver lysate with AMACR antibody. Predicted molecular weight ~43 kDa.
IHC testing of FFPE human prostate carcinoma with AMACR antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free AMACR antibody as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, lambda
Clone Name AMACR/1723
Purity Protein G affinity chromatography
UniProt Q9UHK6
Localization Cytoplasmic
Applications Western blot : 0.5-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This AMACR antibody is available for research use only.
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Description

AMACR, also known as p504S is an enzyme that is involved in bile acid biosynthesis and beta-oxidation of branched-chain fatty acids. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands.

Application Notes

Optimal dilution of the AMACR antibody should be determined by the researcher.

Immunogen

A recombinant human protein was used as the immunogen for the AMACR antibody.

Storage

Store the AMACR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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