AMACR Antibody / Alpha-methylacyl-CoA Racemase Antibody [clone AMACR/1864] (V7479)

AMACR Antibody Renal Carcinoma IHC. Immunohistochemistry staining of FFPE human renal carcinoma tissue using clone AMACR/1864 demonstrates strong diffuse granular cytoplasmic HRP-DAB brown staining in malignant epithelial cells, consistent with the established carcinoma-associated expression pattern of Alpha-methylacyl-CoA racemase / AMACR in renal malignancy tissue. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

AMACR Antibody Prostate Carcinoma IHC. Immunohistochemistry staining of FFPE human prostate carcinoma tissue using clone AMACR/1864 demonstrates granular cytoplasmic HRP-DAB brown staining in malignant prostatic epithelial cells, consistent with the characteristic expression pattern of AMACR / p504S in prostate adenocarcinoma. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

AMACR Antibody PC-3 WB. Western blot analysis of human PC-3 cell lysate using clone AMACR/1864 demonstrates a strong band at approximately 40-43 kDa, consistent with the predicted molecular weight of Alpha-methylacyl-CoA racemase / AMACR. The observed signal supports expression of this carcinoma-associated metabolic enzyme in prostate cancer-derived cells, aligning with the established role of p504S as a prostate adenocarcinoma marker.

AMACR Antibody Human Kidney WB. Western blot analysis of human partial protein and human kidney lysate using clone AMACR/1864 demonstrates a major band at approximately 40-43 kDa in kidney lysate, consistent with the predicted molecular weight of Alpha-methylacyl-CoA racemase / AMACR. The recombinant partial protein control migrates at a lower apparent molecular weight, as expected for a truncated protein fragment used for antibody validation.

AMACR Antibody Protein Microarray Specificity Analysis. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using clone AMACR/1864 identified Alpha-methylacyl-CoA racemase / AMACR as the dominant target with a Z-score of 85.13 and an S-score of 59.47, supporting highly selective target recognition. The markedly elevated S-score demonstrates strong preferential binding of the antibody to AMACR relative to unrelated proteins present on the array. The Z-score represents signal intensity generated by antibody binding relative to the mean signal across the array, while the S-score reflects the difference in signal strength between successive ranked targets, serving as a measure of relative target specificity.

SDS-PAGE analysis of purified, BSA-free AMACR antibody (clone AMACR/1864) as confirmation of integrity and purity.

AMACR Antibody Human Kidney WB. Western blot analysis of human kidney lysate using clone AMACR/1864 demonstrates a distinct band at approximately 40-43 kDa, consistent with the predicted molecular weight of Alpha-methylacyl-CoA racemase / AMACR. The observed signal supports expression of this peroxisomal and mitochondrial metabolic enzyme involved in branched-chain fatty acid and bile acid metabolism within renal tissue.