ALPL Antibody / Phosphate Metabolism Enzyme Antibody (RQ5701)

ALPL Antibody IF. Immunofluorescent staining of FFPE human A431 cells with ALPL Antibody / Phosphate Metabolism Enzyme Antibody demonstrates granular cytoplasmic and cell surface-associated green fluorescence consistent with alkaline phosphatase, tissue-nonspecific isozyme / ALPL localization in epithelial-derived tumor cells. Signal enrichment is observed along the peripheral cytoplasmic compartment surrounding DAPI-stained nuclei (blue), supporting expression of this phosphate metabolism-associated enzyme within metabolically active carcinoma cells. HIER: steam section in pH6 citrate buffer for 20 min.

ALPL Antibody Lung Cancer IHC. Immunohistochemistry analysis of FFPE human lung carcinoma tissue stained with ALPL Antibody / Phosphate Metabolism Enzyme Antibody. Tumor epithelial cells demonstrate diffuse cytoplasmic and membranous HRP-DAB brown staining, while surrounding stromal and inflammatory cell populations show comparatively lower background reactivity. The staining pattern is consistent with expression of alkaline phosphatase, tissue-nonspecific isozyme / ALPL, a phosphate metabolism enzyme associated with epithelial differentiation and metabolic remodeling pathways in tumor tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

ALPL Antibody Rectal Cancer IHC. Immunohistochemistry analysis of FFPE human rectal carcinoma tissue stained with ALPL Antibody / Phosphate Metabolism Enzyme Antibody. Malignant gland-forming epithelial cells demonstrate strong apical membranous and granular cytoplasmic HRP-DAB brown staining, with prominent signal localized along luminal tumor surfaces and differentiated epithelial compartments. The staining profile is consistent with expression of alkaline phosphatase, tissue-nonspecific isozyme / ALPL, a phosphate metabolism enzyme involved in epithelial differentiation and extracellular phosphate regulation pathways. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

ALPL Antibody Rat Brain IHC. Immunohistochemistry analysis of FFPE rat brain tissue stained with ALPL Antibody / Phosphate Metabolism Enzyme Antibody. Scattered neuronal and glial-associated cellular populations demonstrate light to moderate cytoplasmic HRP-DAB brown staining against a low-background neuropil environment. The observed staining pattern is consistent with expression of alkaline phosphatase, tissue-nonspecific isozyme / ALPL, a phosphate metabolism enzyme implicated in extracellular phosphate regulation and neural tissue metabolic homeostasis. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

ALPL Antibody HEK293 WB. Western blot analysis of human HEK293 cell lysate using ALPL Antibody / Phosphate Metabolism Enzyme Antibody detects a prominent band at approximately 72-75 kDa, consistent with glycosylated forms of alkaline phosphatase, tissue-nonspecific isozyme / ALPL. The predicted molecular weight of ALPL is approximately 57 kDa, however the protein is commonly observed at higher apparent molecular weights due to extensive glycosylation and post-translational processing associated with this membrane-bound phosphate metabolism enzyme.

ALPL Antibody HepG2 FACS. Flow cytometry analysis of human HepG2 cells stained with ALPL Antibody / Phosphate Metabolism Enzyme Antibody demonstrates a distinct right-shifted fluorescence population relative to the isotype control, consistent with cell-associated expression of alkaline phosphatase, tissue-nonspecific isozyme / ALPL. This phosphate metabolism enzyme is associated with epithelial differentiation and extracellular phosphate regulation pathways in metabolically active cellular populations. Red=cells alone, Green=isotype control, Blue=ALPL antibody.