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Home >> Antibodies >> Alpha Tubulin Antibody

Alpha Tubulin Antibody [clone TUBA/3038] (V8404)

  Catalog No Formulation Size Price (USD)  
Image V8404-100UG 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 429
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V8404-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V8404SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
IHC staining of FFPE human lung with Alpha Tubulin antibody (clone TUBA/3038). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Alpha Tubulin antibody (clone TUBA/3038). These results demonstrate the foremost specificity of the TUBA/3038 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free Alpha Tubulin antibody (clone TUBA/3038) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name TUBA/3038
Purity Protein G affinity chromatography
UniProt P68363
Localization Cell surface, Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Alpha Tubulin antibody is available for research use only.
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Description

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. This MAb recognizes an epitope of alpha-tubulin. The alpha-tubulin is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain

Application Notes

Optimal dilution of the Alpha Tubulin antibody should be determined by the researcher.

Immunogen

A portion of amino acids 1-115 from the human protein was used as the immunogen for the Alpha Tubulin antibody.

Storage

Store the Alpha Tubulin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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