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Home >> Antibodies >> Alpha Taxilin Antibody / TXLNA

Alpha Taxilin Antibody / TXLNA (RQ7605)

  Catalog No Formulation Size Price (USD)  
Image RQ7605 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 449
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Alpha Taxilin Antibody IF. Immunofluorescent staining of FFPE human PC-3 cells with Alpha Taxilin antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Alpha Taxilin Antibody WB. Western blot testing of human 1) 293T, 2) K562, 3) HepG2, and 4) HeLa cell lysate with Alpha Taxilin antibody. Predicted molecular weight ~62 kDa but commonly observed at ~75 kDa.
Alpha Taxilin Antibody / Vesicle Trafficking Protein Antibody Human and Mouse WB. Western blot analysis of 1) human 293T, 2) human K562, 3) human HepG2, 4) human HeLa, 5) mouse heart, and 6) mouse kidney lysates using Alpha Taxilin Antibody / Vesicle Trafficking Protein Antibody. A specific immunoreactive band is detected at approximately 75 kDa across the tested human and mouse samples, consistent with Alpha Taxilin (TXLNA) expression. The predicted molecular weight of Alpha Taxilin is approximately 62 kDa, with the observed migration reflecting its electrophoretic mobility. These results demonstrate reliable detection of Alpha Taxilin, a cytoplasmic protein involved in vesicle docking, membrane trafficking, and exocytotic processes.
Alpha Taxilin Antibody / Vesicle Trafficking Protein Antibody HepG2 Flow Cytometry. Flow cytometric analysis of fixed and permeabilized human HepG2 cells using Alpha Taxilin Antibody / Vesicle Trafficking Protein Antibody at 1 ug/10^6 cells. Cells were fixed with 4% paraformaldehyde, permeabilized, and blocked with 10% normal goat serum prior to staining. Red: unstained cells. Green: rabbit IgG isotype control. Blue: Alpha Taxilin antibody. The clear rightward shift of the Alpha Taxilin antibody signal relative to the isotype control demonstrates specific intracellular detection of TXLNA in HepG2 cells.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P40222
Localization Cytoplasmic, extracellular
Applications Western Blot : 0.5-1ug/ml
Flow Cytometry : 1-3ug/million cells
Immunofluorescence : 5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This Alpha Taxilin antibody is available for research use only.
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Description

Alpha Taxilin Antibody / TXLNA recognizes alpha taxilin (TXLNA), a ubiquitously expressed cytoplasmic protein that regulates intracellular vesicle trafficking through interactions with members of the syntaxin family of SNARE proteins. Alpha taxilin functions as an important adaptor during membrane fusion and vesicle transport, contributing to the precise delivery of proteins and lipids between intracellular compartments. By modulating syntaxin-dependent trafficking events, TXLNA supports normal secretory activity, membrane organization, and cellular homeostasis. Consequently, Alpha Taxilin Antibody / TXLNA is widely used to investigate membrane trafficking, exocytosis, and intracellular transport pathways.

TXLNA associates with multiple syntaxin isoforms involved in vesicle docking and fusion, allowing it to participate in diverse trafficking processes across many cell types. Through these interactions, alpha taxilin influences protein secretion, receptor recycling, membrane remodeling, and transport between the Golgi apparatus, endosomes, and plasma membrane. Because vesicle trafficking is fundamental to virtually every cell, TXLNA contributes to processes including cell growth, signal transduction, cell migration, and maintenance of tissue architecture.

Altered TXLNA expression has been reported in several malignancies and has been investigated for its potential roles in tumor progression, cellular proliferation, and metastatic behavior. In addition, disruption of vesicle trafficking pathways involving alpha taxilin may contribute to abnormalities in protein secretion and membrane dynamics associated with a variety of pathological conditions. As a result, TXLNA has become an informative marker for studies of membrane transport, intracellular signaling, cancer biology, and secretory pathway regulation.

NSJ Bioreagents' Alpha Taxilin Antibody / TXLNA supports investigations into vesicle trafficking, SNARE-mediated membrane fusion, protein secretion, intracellular transport, receptor recycling, and cancer biology. Reliable detection of TXLNA enables researchers to study an important regulator of syntaxin-dependent membrane trafficking and the cellular processes required for normal membrane organization and secretory function.

Explore additional antibodies involved in vesicle trafficking, membrane transport, and intracellular signaling on our Cell Biology Antibodies page.

Application Notes

Optimal dilution of the Alpha Taxilin antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M1-A546) was used as the immunogen for the Alpha Taxilin antibody.

Storage

After reconstitution, the Alpha Taxilin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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