Alpha-Synuclein Antibody AGA-19 | SNCA Monoclonal

	Catalog No	Formulation	Size	Price (USD)
	RQ8886	Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA	100 ul	449
Formulation

IHC staining of FFPE human colon cancer tissue with Alpha-Synuclein antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Alpha-Synuclein Antibody AGA-19 IHC in Human Glioma. Immunohistochemical staining of FFPE human glioma tissue using the SNCA Monoclonal Antibody clone AGA-19. Brown DAB staining is observed in scattered tumor-associated cells, with prominent cytoplasmic and perinuclear immunoreactivity. Staining is heterogeneous, with some cells exhibiting stronger immunoreactivity while adjacent cells show comparatively limited staining. Heat-induced epitope retrieval was performed in EDTA buffer (pH 8.0) for 20 minutes, followed by detection using an HRP-conjugated secondary antibody and DAB chromogen.

IHC staining of FFPE mouse brain tissue with Alpha-Synuclein antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Alpha-Synuclein Antibody AGA-19 IHC in Mouse Brain. Immunohistochemical staining of FFPE mouse brain tissue using the SNCA Monoclonal Antibody clone AGA-19. Brown DAB staining is prominent in the nuclei of cells within distinct layers of the cerebellar cortex, particularly the densely packed granule cell layer. Additional scattered nuclear staining is observed in the adjacent molecular layer, while the surrounding neuropil exhibits comparatively limited immunoreactivity. Heat-induced epitope retrieval was performed in EDTA buffer (pH 8.0) for 20 minutes, followed by detection using an HRP-conjugated secondary antibody and DAB chromogen.

Alpha-Synuclein Antibody AGA-19 IHC in Rat Brain. Immunohistochemical staining of FFPE rat brain tissue using the SNCA Monoclonal Antibody clone AGA-19. Brown DAB staining is observed throughout the brain parenchyma, with diffuse, finely granular immunoreactivity in the neuropil and more concentrated staining around scattered neuronal cell bodies. The staining exhibits a predominantly cytoplasmic and neuropil-associated distribution, while numerous nuclei remain comparatively unstained. Heat-induced epitope retrieval was performed in EDTA buffer (pH 8.0) for 20 minutes, followed by detection using an HRP-conjugated secondary antibody and DAB chromogen.

Immunofluorescent staining of FFPE human HeLa cells with Alpha-Synuclein antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of human 1) HEL, 2) RT4 and 3) HeLa cell lysate with Alpha-Synuclein antibody. Predicted molecular weight ~15 kDa.

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IHC staining of FFPE human colon cancer tissue with Alpha-Synuclein antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Alpha-Synuclein Antibody AGA-19 IHC in Human Glioma. Immunohistochemical staining of FFPE human glioma tissue using the SNCA Monoclonal Antibody clone AGA-19. Brown DAB staining is observed in scattered tumor-associated cells, with prominent cytoplasmic and perinuclear immunoreactivity. Staining is heterogeneous, with some cells exhibiting stronger immunoreactivity while adjacent cells show comparatively limited staining. Heat-induced epitope retrieval was performed in EDTA buffer (pH 8.0) for 20 minutes, followed by detection using an HRP-conjugated secondary antibody and DAB chromogen.

IHC staining of FFPE mouse brain tissue with Alpha-Synuclein antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Alpha-Synuclein Antibody AGA-19 IHC in Mouse Brain. Immunohistochemical staining of FFPE mouse brain tissue using the SNCA Monoclonal Antibody clone AGA-19. Brown DAB staining is prominent in the nuclei of cells within distinct layers of the cerebellar cortex, particularly the densely packed granule cell layer. Additional scattered nuclear staining is observed in the adjacent molecular layer, while the surrounding neuropil exhibits comparatively limited immunoreactivity. Heat-induced epitope retrieval was performed in EDTA buffer (pH 8.0) for 20 minutes, followed by detection using an HRP-conjugated secondary antibody and DAB chromogen.

Alpha-Synuclein Antibody AGA-19 IHC in Rat Brain. Immunohistochemical staining of FFPE rat brain tissue using the SNCA Monoclonal Antibody clone AGA-19. Brown DAB staining is observed throughout the brain parenchyma, with diffuse, finely granular immunoreactivity in the neuropil and more concentrated staining around scattered neuronal cell bodies. The staining exhibits a predominantly cytoplasmic and neuropil-associated distribution, while numerous nuclei remain comparatively unstained. Heat-induced epitope retrieval was performed in EDTA buffer (pH 8.0) for 20 minutes, followed by detection using an HRP-conjugated secondary antibody and DAB chromogen.

Immunofluorescent staining of FFPE human HeLa cells with Alpha-Synuclein antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of human 1) HEL, 2) RT4 and 3) HeLa cell lysate with Alpha-Synuclein antibody. Predicted molecular weight ~15 kDa.

Species Reactivity	Human, Mouse, Rat
Format	Purified
Host	Rabbit
Clonality	Recombinant Rabbit Monoclonal
Isotype	Rabbit IgG
Clone Name	AGA-19
Purity	Affinity chromatography
UniProt	P37840
Localization	Nuclear, cytoplasmic, cell membrane
Applications	1:500-1:20001:50-1:2001:50-1:200
Limitations	This Alpha-Synuclein Antibody AGA-19 / SNCA Monoclonal Antibody is available for research use only.
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Description

Alpha-Synuclein Antibody AGA-19 / SNCA Monoclonal Antibody recognizes alpha-synuclein, a neuronal protein encoded by the SNCA gene that participates in synaptic vesicle organization, neurotransmitter release, and intracellular membrane dynamics. Alpha-synuclein is a member of the synuclein family, which also includes beta-synuclein and gamma-synuclein. Although especially abundant at presynaptic terminals, alpha-synuclein can associate with multiple cellular membranes, reflecting its ability to interact with lipid surfaces and adopt different structural conformations. These properties contribute to its physiological functions and its involvement in neurodegenerative disease.

Alpha-synuclein is an intrinsically disordered protein whose structure changes upon interaction with lipid membranes. Its N-terminal region contains repeated sequences that promote membrane binding and alpha-helical folding, while the central non-amyloid-beta component region contributes to aggregation propensity. The acidic C-terminal region influences interactions with proteins, metal ions, and other cellular components. Alpha-synuclein has been implicated in synaptic vesicle clustering and the regulation of neurotransmitter release, including interactions with synaptobrevin-2 and other proteins involved in SNARE complex assembly. These activities help coordinate the availability and organization of vesicles at presynaptic terminals.

Under conditions that favor protein misfolding, alpha-synuclein can assemble into oligomers and amyloid fibrils. Aggregated alpha-synuclein is a defining component of Lewy bodies and Lewy neurites, pathological structures associated with Parkinson's disease and dementia with Lewy bodies. Abnormal accumulation also occurs in multiple system atrophy, although the affected cell populations and inclusion characteristics differ. Genetic evidence supports the importance of alpha-synuclein regulation, as SNCA missense mutations and gene multiplications can cause inherited forms of Parkinson's disease. These alterations may influence protein aggregation, membrane interactions, or the balance between synthesis and clearance.

Alpha-synuclein undergoes post-translational modifications that affect its biochemical properties and cellular behavior. Phosphorylation at Ser129 is strongly associated with pathological inclusions, while ubiquitination, nitration, and proteolytic processing have also been investigated in relation to aggregation and clearance. Cellular pathways involving autophagy, lysosomal degradation, and mitochondrial function influence alpha-synuclein homeostasis. Misfolded assemblies may also promote the conversion of soluble alpha-synuclein into aggregated forms, contributing to the spread of pathology through interconnected cellular populations. The Alpha-Synuclein Antibody AGA-19 provides a clone-defined reagent for examining alpha-synuclein expression in relation to synaptic biology, protein aggregation, and mechanisms of neurodegeneration.

Explore our SNCA Antibody (FY12837) for additional research into alpha-synuclein expression, synaptic function, and neurodegenerative disease mechanisms, with Western blot validation using HeLa wild-type and SNCA knockout cell lysates.

Application Notes

Optimal dilution of the Alpha-Synuclein Antibody AGA-19 / SNCA Monoclonal Antibody should be determined by the researcher.

Immunogen

A peptide sequence specific to Alpha-Synuclein was used as the immunogen for the Alpha-Synuclein antibody.

Storage

After reconstitution, the Alpha-Synuclein antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

Alpha synuclein antibody, Synuclein alpha antibody, SNCA antibody, Alpha-synuclein protein antibody, Non-amyloid beta component precursor antibody, NACP antibody, Parkinson disease protein 1 antibody, Synuclein family alpha protein antibody

Alpha-Synuclein Antibody AGA-19 / SNCA Monoclonal Antibody [clone AGA-19] (RQ8886)

Description

Application Notes

Immunogen

Storage

Alternate Names

Area of Research

Product Type

Protocols

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