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Home >> Antibodies >> Alpha Internexin Antibody

Alpha Internexin Antibody (R32440)

  Catalog No Formulation Size Price (USD)  
Image R32440 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of 1) rat brain, 2) mouse brain and 3) human 22RV1 lysate with Alpha Internexin antibody at 0.5ug/ml. Predicted molecular weight ~55 kDa but routinely observed at ~66 kDa.
IHC testing of FFPE human glioma tissue with HMGB2 antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
IHC testing of FFPE mouse brain with HMGB2 antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
IHC testing of FFPE rat brain with HMGB2 antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt Q16352
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 1-2ug/ml
Limitations This Alpha Internexin antibody is available for research use only.
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Description

Alpha-Internexin (INA; also NF-66) is a 66 kDa member of the intermediate filament (IF) protein family. The protein was originally purified from rat optic nerve and spinal cord. And the protein copurifies with other neurofilament subunits, as it was originally discovered, however in some mature neurons it can be the only neurofilament expressed. The protein is present in developing neuroblasts and in the Central Nervous System of adults. Meanwhile, the protein is a major component of the intermediate filament network in small interneurons and cerebellar granule cells, where it is present in the parallel fibers.

Application Notes

Optimal dilution of the Alpha Internexin antibody should be determined by the researcher.

Immunogen

Amino acids A71-R161 were used as the immunogen for the Alpha Internexin antibody.

Storage

Prior to reconstitution, store at 4oC. After reconstitution, the Alpha Internexin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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