Alpha Internexin Antibody / INA / Neurofilament-66 (R32440)

Immunofluorescent staining of FFPE mouse brain tissue with Alpha Internexin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Immunofluorescent staining of FFPE rat brain tissue with Alpha Internexin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Immunofluorescent staining of FFPE human A549 cells with Alpha Internexin antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

IHC staining of FFPE rat brain tissue with Alpha Internexin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat brain tissue with Alpha Internexin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse brain tissue with Alpha Internexin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human glioma tissue with Alpha Internexin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of 1) human SH-SY5Y, 2) human A549, 3) human HeLa, 4) rat brain, 5) rat C6, 6) mouse brain and 7) mouse Neuro-2a cell lysate with Alpha Internexin antibody. Predicted molecular weight ~55 kDa but routinely observed at ~66 kDa.

Flow cytometry testing of fixed and permeabilized human SiHa cells with Alpha Internexin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Alpha Internexin antibody.