Alpha II Spectrin Antibody / Alpha Fodrin / SPTAN1 / NEAS [clone SPTAN1/3352] (V8096)

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SPTAN1 antibody (clone SPTAN1/3352). These results demonstrate the foremost specificity of the SPTAN1/3352 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Immunohistochemistry of Alpha II Spectrin antibody in human lung tissue. FFPE human lung shows cytoplasmic HRP-DAB brown staining in epithelial and inflammatory cells, consistent with SPTAN1 localization along the submembranous cytoskeleton. Clone SPTAN1/3352 was used following heat-induced epitope retrieval by boiling tissue sections in pH 6, 10 mM citrate buffer for 20 minutes and allowing sections to cool prior to staining.

Alpha II Spectrin antibody IHC staining of FFPE human lung tissue using clone SPTAN1/3352. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Alpha II Spectrin antibody (clone SPTAN1/3352) as confirmation of integrity and purity.