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Home >> Antibodies >> Alpha Fetoprotein Antibody / AFP

Alpha Fetoprotein Antibody / AFP (RQ7141)

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  Catalog No Formulation Size Price (USD)  
Image RQ7141 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human 1) HepG2 and 2) HCCP cell lysate with Alpha Fetoprotein antibody. Predicted molecular weight: ~70 kDa.
Western blot testing of human 1) HepG2 and 2) HCCP cell lysate with Alpha Fetoprotein antibody. Predicted molecular weight: ~70 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P02771
Applications Western blot : 0.5-1ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This Alpha Fetoprotein antibody is available for research use only.
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Description

AFP, also called Alpha-fetoprotein, is a protein that in humans is encoded by the AFP gene. It is mapped to 4q13.3. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. In rodents AFP binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. Moreover, it has an important role as a diagnostic marker, especially in certain tumors and liver diseases of childhood. AFP is also used to test the potential usefulness of plasma alpha fetoprotein determination as a diagnostic marker in biliary atresia, hepatitis, and yolk sac derived tumours.

Application Notes

Optimal dilution of the Alpha Fetoprotein antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids R19-L258) was used as the immunogen for the Alpha Fetoprotein antibody.

Storage

After reconstitution, the Alpha Fetoprotein antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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