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Home >> Antibodies >> Alpha Catenin Antibody

Alpha Catenin Antibody [clone 1G5] (V3202)

  Catalog No Formulation Size Price (USD)  
Image V3202-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V3202-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V3202SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
Western blot testing of human 1) A431 and 2) MCF7 cell lysate with Alpha Catenin antibody (clone 1G5). Expected molecular weight ~102 kDa.
SDS-PAGE Analysis of Purified, BSA-Free Alpha Catenin Antibody (clone 1G5). Confirmation of Integrity and Purity of the Antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name 1G5
Purity Protein G affinity chromatography
UniProt P35221
Localization Cell surface, cytoplasmic, cell junctions
Applications Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Western blot : 0.5-1ug/ml
Limitations This Alpha Catenin antibody is available for research use only.
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Description

Recognizes a protein of 102kDa, identified as Catenin, alpha-1. Catenins comprise a large family of Ca2+-dependent, homotypic cell-cell adhesion molecules that play important roles in development, epithelial cell polarity and tumor progression. Alpha-Catenin is a key regulator of actin dynamics in cell-cell adhesion. During cell-cell adhesion, alpha-Catenin forms a heterodimer with beta-Catenin and links the cadherins to actin associated with the cytoskeleton. Alpha-Catenin also regulates the beta-Catenin signaling in various cells. It displays the tumor suppressor activity and is found to be down regulated in some forms of breast cancer.

Application Notes

Optimal dilution of the Alpha Catenin antibody to be determined by the researcher.

Immunogen

A human fusion protein was used as the immunogen for the Alpha Catenin antibody.

Storage

Store the Alpha Catenin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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