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Home >> Antibodies >> Alpha-Adaptin Antibody

Alpha-Adaptin Antibody [clone 100/2] (R30093)

  Catalog No Formulation Size Price (USD)  
Image R30093 0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS 100 ug 329
Availability 1-3 business days
Species Reactivity Human, Rat
Format Ascites
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a
Clone Name 100/2
Purity Ascites
Gene ID 160
Applications Western blot : 2-4ug/ml
Limitations This alpha-Adaptin antibody is available for research use only.
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Description

Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1(HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al.(2001) identified 3 HIP1-associated proteins, clathrin heavy chain(CLTC)and alpha-adaptin A and C. Coat proteins of approximately 100-kD(adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the alpha-Adaptin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

AP-2 adaptor polypeptides from bovine brain was used as the immunogen for this alpha-Adaptin antibody.

Storage

After reconstitution, the alpha-Adaptin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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