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Home >> Antibodies >> Alpha Actin Antibody / ACATA1

Alpha Actin Antibody / ACATA1 [clone C-1] (RQ4494)

  Catalog No Formulation Size Price (USD)  
Image RQ4494 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 419
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Western blot testing of human 1) A431 and 2) MCF7 lysate with Alpha Actin antibody at 0.5ug/ml. Expected molecular weight: 42-45 kDa.
IHC testing of FFPE mouse muscle with Alpha Actin antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Immunofluorescent staining of human HeLa cells with Alpha Actin antibody (green) and DAPI nuclear stain (blue).
Availability 1-2 weeks
Species Reactivity Human, Mouse
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name C-1
Purity Protein A affinity
UniProt P68133
Localization Cytoplasm, cytoskeleton
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:50-1:200
Immunofluorescence : 1:50-1:200
Limitations This Alpha Actin antibody is available for research use only.
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Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Application Notes

Optimal dilution of the Alpha Actin antibody should be determined by the researcher.


A synthetic peptide from human Alpha Actin was used as the immunogen for the Alpha Actin antibody.


Store the Alpha Actin antibody at -20oC.

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