Alpha Actin Antibody | ACTA2 | Smooth Muscle Actin

	Catalog No	Formulation	Size	Price (USD)
	RQ8953	Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA	100 ul	439
Formulation

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Immunofluorescent staining of FFPE human A431 cells with Alpha Actin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunofluorescent staining of FFPE human HeLa cells with Alpha Actin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

IHC staining of FFPE human breast carcinoma tissue with Alpha Actin antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of 1) human A431, 2) human A549, 3) human HeLa, 4) rat C6 and 5) mouse NIH 3T3 cell lysate using Alpha Actin antibody. Predicted molecular weight ~42 kDa.

Availability	1-2 weeks
Species Reactivity	Human, Mouse, Rat
Format	Purified
Clonality	Recombinant Rabbit Monoclonal
Isotype	Rabbit IgG
Clone Name	CAD-1
Purity	Affinity purified
UniProt	P62736
Localization	Cytoplasmic
Applications	Western Blot : 1:500 Immunohistochemistry (FFPE) : 1:50 Immunofluorescence : 1:50
Limitations	This Alpha Actin antibody is available for research use only.
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Description

Alpha Actin antibody is a key reagent for studying cytoskeletal dynamics, smooth muscle physiology, and tissue remodeling. The encoded protein, ACTA2 (actin alpha 2), is a smooth muscle specific isoform of actin that contributes to contraction, structure, and motility of vascular and visceral smooth muscle cells. As a fundamental component of the cytoskeleton, ACTA2 polymerizes into filaments that provide mechanical strength and coordinate intracellular signaling.

Alpha actin is highly expressed in the smooth muscle layers of blood vessels, the respiratory tract, the gastrointestinal system, and reproductive organs. It also serves as a well established marker for myofibroblasts, which emerge during wound healing and fibrotic disease. Upregulation of ACTA2 in fibroblasts indicates their transition into contractile myofibroblasts, a process central to tissue repair and pathologic fibrosis. Detection with Alpha Actin antibody allows researchers to track these changes in both normal physiology and disease progression.

In cardiovascular research, ACTA2 is examined as a marker of vascular smooth muscle cells and their response to injury or remodeling. Phenotypic switching of smooth muscle cells, from a contractile to a synthetic state, is associated with vascular disease such as atherosclerosis. In liver, kidney, and lung fibrosis, ACTA2 positive myofibroblasts accumulate and contribute to excessive extracellular matrix deposition. Because of this, the Alpha Actin antibody is routinely applied in histological studies of fibrotic disorders.

At the molecular level, ACTA2 shares high sequence similarity with other actin isoforms but contains unique features that confer smooth muscle specific function. It interacts with actin binding proteins to regulate filament turnover, contractility, and cell migration. The strong conservation of actin family proteins ensures that Alpha Actin antibody provides reliable detection across species in research models.

The Alpha Actin antibody is widely used in immunohistochemistry, immunofluorescence, western blotting, and flow cytometry. These applications enable localization and quantification of ACTA2 in tissue samples and cultured cells. For researchers focused on vascular biology, tissue fibrosis, or cytoskeletal organization, the Alpha Actin antibody is a specific and dependable detection tool. NSJ Bioreagents offers validated antibodies to ensure reproducibility and accuracy in molecular and cellular studies.

Application Notes

Optimal dilution of the Alpha Actin antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human ACTA2 protein was used as the immunogen for the Alpha Actin antibody.

Storage

Store the Alpha Actin antibody at -20oC.

Alpha Actin Antibody / ACTA2 / Smooth Muscle Actin [clone CAD-1] (RQ8953)

Description

Application Notes

Immunogen

Storage

Area of Research

Product Type

Protocols

Contact Us

NSJ Bioreagents