Alpha A Crystallin Antibody / Molecular Chaperone Antibody (R32408)

Alpha A Crystallin Antibody Human, Mouse, Rat WB. Western blot analysis of Alpha A Crystallin expression was performed using anti-Alpha A Crystallin (CRYAA) antibody. Lane 1: rat spleen lysate. Lane 2: mouse eye lysate. Lane 3: human SMMC-7721 cell lysate. Alpha A Crystallin is a small heat shock protein and ATP-independent molecular chaperone that maintains protein homeostasis by preventing protein aggregation and protecting cells from stress-induced damage. A specific immunoreactive band is detected at approximately 20–23 kDa in all samples, consistent with the expected molecular weight of Alpha A Crystallin. These results demonstrate cross-species reactivity and support the utility of Alpha A Crystallin Antibody for studies of molecular chaperone function, protein homeostasis, ocular biology, and cellular stress responses.

Alpha A Crystallin Antibody Mouse Spleen IHC. IHC staining of FFPE mouse spleen with Alpha A Crystallin antibody demonstrated cytoplasmic staining in scattered splenic cells. Alpha A Crystallin (CRYAA) is a small heat shock protein and ATP-independent molecular chaperone that protects cells from protein aggregation, oxidative stress, and apoptosis while maintaining protein homeostasis. Although best known as a major structural protein of the ocular lens, Alpha A Crystallin is also expressed in selected non-lenticular tissues where it contributes to cellular stress responses and cytoprotection. HIER was performed by boiling tissue sections in pH 6, 10 mM citrate buffer for 20 minutes followed by cooling prior to staining. These results support the utility of Alpha A Crystallin Antibody for studies of molecular chaperone function, protein homeostasis, and cellular stress responses.

Alpha A Crystallin Antibody Rat Spleen IHC. IHC staining of FFPE rat spleen with Alpha A Crystallin antibody demonstrated cytoplasmic staining in scattered splenic cells. Alpha A Crystallin (CRYAA) is a small heat shock protein and ATP-independent molecular chaperone that protects cells from protein aggregation, oxidative stress, and apoptosis while maintaining protein homeostasis. Although predominantly expressed in the ocular lens, Alpha A Crystallin is also present in selected non-lenticular tissues where it contributes to cellular stress responses and cytoprotection. HIER was performed by boiling tissue sections in pH 6, 10 mM citrate buffer for 20 minutes followed by cooling prior to staining. These results support the utility of Alpha A Crystallin Antibody for studies of molecular chaperone function, protein homeostasis, and cellular stress responses.

Alpha A Crystallin Antibody Human HepG2 Flow Cytometry. Flow cytometric analysis of human HepG2 cells using Alpha A Crystallin antibody demonstrated a clear rightward shift in fluorescence intensity (blue) compared with the isotype control (green) and unstained cells (red), indicating specific detection of Alpha A Crystallin expression. Alpha A Crystallin (CRYAA) is a small heat shock protein and ATP-independent molecular chaperone that protects cells from protein aggregation, oxidative stress, and apoptosis while maintaining protein homeostasis. HepG2 cells were incubated with Alpha A Crystallin antibody at 1 ug/10^6 cells following goat serum blocking. These results support the utility of Alpha A Crystallin Antibody for flow cytometric studies of molecular chaperone function, protein homeostasis, and cellular stress responses.