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Home >> Antibodies >> Alpha 1 microglobulin Antibody / AMBP

Alpha 1 microglobulin Antibody / AMBP (RQ4535)

  Catalog No Formulation Size Price (USD)  
Image RQ4535 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of human 1) placenta, 2) HEp-2, 3) HeLa, 4) A549 and 5) PANC-1 lysate with Alpha 1 microglobulin antibody at 0.5ug/ml. Predicted molecular weight: 26-28 kDa (Alpha 1 microglobulin), ~39 kDa (uncleaved AMBP). These proteins may be observed at higher molecular weights due to glycosylation.
IHC staining of FFPE human liver cancer with Alpha 1 microglobulin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human liver cancer with Alpha 1 microglobulin antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P02760
Localization Secreted
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 1-2ug/ml
Direct ELISA : 0.1-0.5ug/ml (human recombinant protein)
Limitations This Alpha 1 microglobulin antibody is available for research use only.
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Description

Protein AMBP is a protein that in humans is encoded by the AMBP gene. This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes.

Application Notes

Optimal dilution of the Alpha 1 microglobulin antibody should be determined by the researcher.

Immunogen

Amino acids G20-V203 from the human protein were used as the immunogen for the Alpha 1 microglobulin antibody.

Storage

After reconstitution, the Alpha 1 microglobulin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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