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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Alpha 1 Antitrypsin Antibody / Hepatocyte Marker [clone AAT/1378] (V3221)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V3221-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
|
|
V3221-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
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V3221SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | AAT/1378 |
| Purity | Protein G affinity |
| UniProt | P01009 |
| Localization | Cytoplasmic |
| Applications | Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT |
| Limitations | This Alpha 1 Antitrypsin antibody is available for research use only. |
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Alpha 1 Antitrypsin antibody, also referred to as a Hepatocyte Marker antibody, recognizes a secreted serine protease inhibitor encoded by the SERPINA1 gene on chromosome 14q32.13. Alpha 1 Antitrypsin, commonly abbreviated A1AT and also known as alpha-1 proteinase inhibitor, is a member of the clade A serpin family. It is synthesized primarily by hepatocytes and secreted into the bloodstream, where it functions as a major inhibitor of neutrophil elastase and other proteolytic enzymes. Within tissue sections, Alpha 1 Antitrypsin is typically localized to the cytoplasm of hepatocytes, making it a widely used marker of hepatocellular differentiation.
As a Hepatocyte Marker, Alpha 1 Antitrypsin antibody is frequently used in liver pathology research to identify hepatocytes in normal and neoplastic tissue. Strong cytoplasmic staining is characteristic of hepatocellular lineage, and expression can assist in distinguishing hepatocellular tumors from metastatic lesions in diagnostic research settings. Beyond the liver, A1AT may be detected in macrophages and certain epithelial cells under inflammatory conditions.
Functionally, Alpha 1 Antitrypsin protects tissues from protease-mediated injury by forming irreversible complexes with target enzymes. In the lung, it plays a critical role in preserving alveolar structure by neutralizing neutrophil elastase. Structurally, the protein contains a reactive center loop that enables protease inhibition and is glycosylated to support stability and secretion. Mutations in SERPINA1 can result in misfolded protein accumulation within hepatocytes, leading to intracellular inclusions and reduced circulating levels.
Deficiency or dysfunctional variants of Alpha 1 Antitrypsin are associated with emphysema, chronic obstructive pulmonary disease, and liver diseases including hepatitis and cirrhosis. Because of its strong hepatocyte-specific cytoplasmic expression pattern, Alpha 1 Antitrypsin antibody serves as a reliable Hepatocyte Marker in studies of liver development, regeneration, and tumor biology. Clone AAT/1378 recognizes Alpha 1 Antitrypsin and is suitable for detecting A1AT expression in relevant research applications.
The stated application concentrations are suggested starting amounts. Titration of the Alpha 1 Antitrypsin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A human partial recombinant protein was used as the immunogen for this Alpha 1 Antitrypsin antibody.
Store the Alpha 1 Antitrypsin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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