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Home >> Antibodies >> Alpha 1 Antitrypsin Antibody (Hepatocyte Marker)

Alpha 1 Antitrypsin Antibody (Hepatocyte Marker) [clone AAT/1378] (V3221)

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  Catalog No Formulation Size Price (USD)  
Image V3221-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3221-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3221SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC testing of FFPE human tonsil with Alpha 1 Antitrypsin antibody (clone AAT/1378). Required HIER: steam sections in pH6, 10mM citrate buffer for 10-20 min.
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IHC testing of FFPE human tonsil with Alpha 1 Antitrypsin antibody (clone AAT/1378). Required HIER: steam sections in pH6, 10mM citrate buffer for 10-20 min.
Western blot testing of 1) a partial recombinant protein, 2) human Jurkat and 3) human A549 lysate with Alpha 1 Antitrypsin antibody (clone AAT/1378). Expected molecular weight: ~47 kDa (unmodified), 52 kDa (glycosylated).
SDS-PAGE Analysis of Purified, BSA-Free Alpha 1 Antitrypsin Antibody (clone AAT/1378). Confirmation of Integrity and Purity of the Antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name AAT/1378
Purity Protein G affinity
UniProt P01009
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This Alpha 1 Antitrypsin antibody is available for research use only.
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Description

The immunohistochemical staining of Alpha 1 Antitrypsin (AAT) is useful in identification of benign and malignant hepatic tumors and yolk sac carcinomas. Positive staining for AAT is also used in detection of benign and malignant lesions of histiocytic nature. This antibody is may also useful tool in the screening of patients with cryptogenic cirrhosis or other forms of liver disease with fibrosis of uncertain origin.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Alpha 1 Antitrypsin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A human partial recombinant protein was used as the immunogen for this Alpha 1 Antitrypsin antibody.

Storage

Store the Alpha 1 Antitrypsin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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