Alpha-1-Antitrypsin Antibody / A1AT / SERPINA1 [clone AAT/4609] (V4364)

Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using Alpha-1-Antitrypsin antibody (clone AAT/4609). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

IHC staining of FFPE human kidney carcinoma tissue with Alpha-1-Antitrypsin antibody (clone AAT/4609). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human lung tissue lysate with Alpha-1-Antitrypsin antibody. Expected molecular weight: 47-52 kDa depending on the level of glycosylation.

SDS-PAGE analysis of purified, BSA-free Alpha-1-Antitrypsin antibody (clone AAT/4609) as confirmation of integrity and purity.