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Home >> Antibodies >> Alpha-1-Antichymotrypsin Antibody / AACT / SERPINA3

Alpha-1-Antichymotrypsin Antibody / AACT / SERPINA3 (RQ7626)

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  Catalog No Formulation Size Price (USD)  
Image RQ7626 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human HepG2 cells with Alpha-1-Antichymotrypsin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Alpha-1-Antichymotrypsin antibody.
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Flow cytometry testing of human HepG2 cells with Alpha-1-Antichymotrypsin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Alpha-1-Antichymotrypsin antibody.
Western blot testing of human 1) HepG2, 2) HCCT and 3) HUH-7 cell lysate with Alpha-1-Antichymotrypsin antibody. Expected molecular weight: 47-65 kDa, depending on level of glycosylation.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P01011
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Alpha-1-Antichymotrypsin antibody is available for research use only.
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Description

Alpha 1-antichymotrypsin, also called serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 or GIG24 is an alpha globulin glycoprotein that is a member of the serpin superfamily. In humans, it is encoded by the SERPINA3 gene. This gene is mapped to 14q32.13. The protein encoded by this gene is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease.

Application Notes

Optimal dilution of the Alpha-1-Antichymotrypsin antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids H24-A423) was used as the immunogen for the Alpha-1-Antichymotrypsin antibody.

Storage

After reconstitution, the Alpha-1-Antichymotrypsin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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