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Home >> Antibodies >> ALKBH5 Antibody

ALKBH5 Antibody (RQ5957)

  Catalog No Formulation Size Price (USD)  
Image RQ5957 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 359
IHC staining of FFPE human breast cancer with ALKBH5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HeLa cells with ALKBH5 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) Caco-2, 2) HeLa, 3) HepG2, 4) U-2 OS and 5) PANC-1 lysate with ALKBH5 antibody. Predicted molecular weight: ~52 kDa.
Flow cytometry testing of human A431 cells with ALKBH5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ALKBH5 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q6P6C2
Localization Nuclear, cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry : 1-2ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This ALKBH5 antibody is available for research use only.
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Description

RNA demethylase ALKBH5 is an enzyme that in humans is encoded by the ALKBH5 gene. It is mapped to 17p11.2. N(6)-methyladenosine (m(6)A) is the most prevalent modification of mRNA. ALKBH5 functions as a demethylase that removes m(6)A from mRNAs.

Application Notes

Optimal dilution of the ALKBH5 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids E81-R180) was used as the immunogen for the ALKBH5 antibody.

Storage

After reconstitution, the ALKBH5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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