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Home >> Antibodies >> ALKBH5 Antibody

ALKBH5 Antibody (RQ5957)

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  Catalog No Formulation Size Price (USD)  
Image RQ5957 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 419
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Immunofluorescent staining of FFPE human A549 cells with ALKBH5 antibody (orange) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human A549 cells with ALKBH5 antibody (orange) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) 293T, 2) Jurkat, 3) HeLa, 4) HepG2 and 5) U-87 MG cell lysate with ALKBH5 antibody. Predicted molecular weight: ~52 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q6P6C2
Localization Nuclear, cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This ALKBH5 antibody is available for research use only.
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  • Applications : WB, FACS, IHC-P
    Reactivity : Human

Description

RNA demethylase ALKBH5 is an enzyme that in humans is encoded by the ALKBH5 gene. It is mapped to 17p11.2. N(6)-methyladenosine (m(6)A) is the most prevalent modification of mRNA. ALKBH5 functions as a demethylase that removes m(6)A from mRNAs.

Application Notes

Optimal dilution of the ALKBH5 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids E81-R180) was used as the immunogen for the ALKBH5 antibody.

Storage

After reconstitution, the ALKBH5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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