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Home >> Antibodies >> Alkaline Phosphatase Antibody (tissue-nonspecific)

Alkaline Phosphatase Antibody (tissue-nonspecific) (R30169)

  Catalog No Formulation Size Price (USD)  
Image R30169 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of Alkaline Phosphatase antibody at 0.5ug/ml and human HT1080 lysate. Predicted molecular weight ~57 kDa, but can be observed at up to ~75 kDa due to glycosylation.
IHC-P: Alkaline Phosphatase antibody testing of rat small intestine tissue
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P05186
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This Alkaline Phosphatase antibody is available for research use only.
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Description

Alkaline phosphatase, tissue-nonspecific isozyme is an enzyme that in humans is encoded by the ALPL gene. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. Liver/bone/kidney alkaline phosphatase, known as tissue-nonspecific, acts physiologically as a lipid-anchored phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP) ectophosphatase. Mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to a disorder known as hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects.

Application Notes

Titration of the Alkaline Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids 21-35 (EKEKDPKYWRDQAQE) from human ALPL were used as the immunogen for this tissue-nonspecific Alkaline Phosphatase antibody.

Storage

The lyophilized Alkaline Phosphatase antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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