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Home >> Antibodies >> Alkaline Phosphatase Antibody (placental)

Alkaline Phosphatase Antibody (placental) [clone 421CT8.1.1] (F40345)

  Catalog No Formulation Size Price (USD)  
Image F40345-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 329
Image
F40345-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
Alkaline Phosphatase antibody western blot analysis in HeLa lysate. Predicted size 60~70 kDa
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name 421CT8.1.1
Purity Purified
UniProt P05187
Applications Western blot : 1:100-1:500
Limitations This Alkaline Phosphatase antibody is available for research use only.
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Description

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. Placental Alkaline Phosphatase (ALPP) is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.

Application Notes

Titration of the Alkaline Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 56-83 from human ALPP was used as the immunogen for this Alkaline Phosphatase antibody.

Storage

Aliquot the Alkaline Phosphatase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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