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Home >> Antibodies >> ALK2 Antibody / ACVR1

ALK2 Antibody / ACVR1 (F54674)

  Catalog No Formulation Size Price (USD)  
Image F54674-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 359
Image
F54674-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 149
Western blot testing of mouse bladder tissue lysate with ALK2 antibody. Predicted molecular weight ~57 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with ALK2 antibody. Predicted molecular weight ~57 kDa.
Western blot testing of human HeLa cell lysate with ALK2 antibody. Predicted molecular weight ~57 kDa.
IHC testing of FFPE mouse skeletal muscle tissue with ALK2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt P37172
Applications Immunohistochemistry (FFPE) : 1:25
Western blot : 1:500-1:2000
Limitations This ALK2 antibody is available for research use only.
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Description

On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin. May be involved in left-right pattern formation during embryogenesis.

Application Notes

The stated application concentrations are suggested starting points. Titration of the ALK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 137-166 from the mouse protein was used as the immunogen for the ALK2 antibody.

Storage

Aliquot the ALK2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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