ALK Antibody / Anaplastic lymphoma kinase (R30831)

ALK Antibody Rectal Adenocarcinoma IHC. Immunohistochemistry staining of FFPE human rectal carcinoma tissue using ALK antibody demonstrates membranous and apical cytoplasmic HRP-DAB brown staining within malignant glandular epithelial cells. The staining pattern highlights polarized epithelial architecture and supports detection of ALK-associated signaling protein expression in colorectal adenocarcinoma-derived tumor tissue. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.

ALK Antibody Rat Intestinal Epithelium IHC. Immunohistochemistry staining of FFPE rat intestine using ALK antibody demonstrates predominantly nuclear and apical-associated HRP-DAB brown staining within intestinal epithelial cells lining mucosal glandular structures and villus-associated compartments. The observed staining pattern supports expression of ALK-associated signaling protein within gastrointestinal epithelial tissue. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.

ALK Antibody Mouse Brain IHC. Immunohistochemical staining of FFPE mouse brain tissue with ALK antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed in pH 8 EDTA buffer prior to staining. ALK immunoreactivity is observed predominantly in neuronal cell bodies and processes, consistent with the expected expression of Anaplastic Lymphoma Kinase (ALK), a receptor tyrosine kinase that regulates neuronal development, cell differentiation, and intracellular signaling pathways. Cell nuclei are counterstained with hematoxylin (blue).

ALK Antibody Rat Brain IHC. Immunohistochemical staining of FFPE rat brain tissue with ALK antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed in pH 8 EDTA buffer prior to staining. ALK immunoreactivity is observed predominantly in neuronal cell bodies and processes, consistent with the expected expression of Anaplastic Lymphoma Kinase (ALK), a receptor tyrosine kinase that regulates neuronal development, neuronal differentiation, and intracellular signaling pathways. Cell nuclei are counterstained with hematoxylin (blue).

ALK Antibody SH-SY5Y Cell WB. Western blot testing of human SH-SY5Y cell lysate with ALK antibody at 0.5 ug/ml. A distinct immunoreactive band is detected at approximately 250 kDa, consistent with the mature glycosylated form of Anaplastic Lymphoma Kinase (ALK). Although the predicted core molecular weight is approximately 176 kDa, ALK commonly migrates at a higher apparent molecular weight due to extensive post-translational glycosylation. This receptor tyrosine kinase regulates neuronal development, cell differentiation, and intracellular signaling pathways.