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Home >> Antibodies >> ALK Antibody / Anaplastic Lymphoma Kinase

ALK Antibody / Anaplastic Lymphoma Kinase [clone ATKR-1] (V3818)

  Catalog No Formulation Size Price (USD)  
Image V3818-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V3818-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V3818SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
IHC testing of FFPE anaplastic large-cell lymphoma with ALK antibody (clone ATKR-1). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name ATKR-1
Purity Protein G affinity
UniProt Q9UM73
Localization Cytoplasmic, nuclear
Applications IHC (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This ALK antibody is available for research use only.
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Description

Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK. [UniProt]

Application Notes

Optimal dilution of the ALK antibody should be determined by the researcher.

Immunogen

A portion of amino acids 200-335 from the human protein was used as the immunogen for this ALK antibody.

Storage

Store the ALK antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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