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Home >> Antibodies >> ALK Antibody / Anaplastic Lymphoma Kinase

ALK Antibody / Anaplastic Lymphoma Kinase [clone ALK/1504] (V3817)

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  Catalog No Formulation Size Price (USD)  
Image V3817-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3817-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3817SAF-100UG 0.7 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC testing of FFPE anaplastic large-cell lymphoma with ALK antibody (clone ALK/1504). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE anaplastic large-cell lymphoma with ALK antibody (clone ALK/1504). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE Ewing's Sarcoma with ALK antibody (clone ALK/1504). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free ALK antibody (clone ALK/1504) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name ALK/1504
Purity Protein G affinity
UniProt Q9UM73
Localization Cytoplasmic, nuclear
Applications Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This ALK antibody is available for research use only.
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Description

The wild-type anaplastic lymphoma kinase (ALK) protein is a 200kDa transmembrane receptor tyrosine kinase. Its expression is restricted to a few scattered cells in the nervous system (some glial cells and neurons, and a few endothelial cells and pericytes. The hybrid gene, NPM-ALK, created by the t(2;5)(p23;q35) chromosomal translocation encodes part of the nucleolar phosphoprotein, nucleophosmin (NPM), joined to the entire cytoplasmic portion of the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. As a consequence, the ALK gene comes under the control of the NPM promoter, which induces a permanent and ubiquitous transcription of the NPM-ALK hybrid gene, resulting in the production of a 80kDa NPM-ALK chimeric protein. This translocation is found in anaplastic large cell lymphomas (ALCL). Reportedly, expression of ALK indicates a better prognosis. Approximately 5%-10% of non-small cell lung carcinomas also express ALK protein producing a cytoplasmic staining pattern. This MAb also reacts with blood vessels that serves as an internal positive control.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the ALK antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 200-335 from the human protein was used as the immunogen for this ALK antibody.

Storage

Store the recombinant ALK antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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