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Home >> Antibodies >> ALDH3 Antibody / ALDH3A1 / Aldehyde dehydrogenase 3

ALDH3 Antibody / ALDH3A1 / Aldehyde dehydrogenase 3 (RQ7035)

  Catalog No Formulation Size Price (USD)  
Image RQ7035 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 419
Flow cytometry testing of human RT4 cells with ALDH3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ALDH3 antibody.
IHC staining of FFPE human rectal cancer tissue with ALDH3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human A549 cell lysate with ALDH3 antibody. Predicted molecular weight: ~51 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P30838
Applications Western blot : 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This ALDH3 antibody is available for research use only.
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Description

Aldehyde dehydrogenase, dimeric NADP-preferring is an enzyme that in humans is encoded by the ALDH3A1 gene. Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The enzyme encoded by this gene forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Application Notes

Optimal dilution of the ALDH3 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids E62-H101) was used as the immunogen for the ALDH3 antibody.

Storage

After reconstitution, the ALDH3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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