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Home >> Antibodies >> ALDH2 Antibody

ALDH2 Antibody [clone 138CT22.3.8] (F40217)

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  Catalog No Formulation Size Price (USD)  
Image F40217-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F40217-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of lysate from NCI-H292, A549, HepG2 cell line using ALDH2 antibody at 1:1000 for each lane. Predicted molecular weight: ~56kDa.
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Western blot analysis of lysate from NCI-H292, A549, HepG2 cell line using ALDH2 antibody at 1:1000 for each lane. Predicted molecular weight: ~56kDa.
ALDH2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue.
ALDH2 antibody flow cytometric analysis of HepG2 cells (red) compared to a negative control cell (blue). PE-conjugated goat-anti-mouse secondary Ab was used for the analysis.
Confocal immunofluorescent analysis of ALDH2 antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used as a nuclear counterstain (blue).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, k
Clone Name 138CT22.3.8
Purity Purified
UniProt P05091
Applications Western blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Flow Cytometry : 1:10-1:50
Immunofluorescence : 1:10-1:50
Limitations This ALDH2 antibody is available for research use only.
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Description

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix.

Application Notes

Titration of the ALDH2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This ALDH2 antibody was produced from a mouse immunized with ALDH2 recombinant protein.

Storage

Aliquot the ALDH2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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