ALDH1A1 Antibody / Cancer Stem Cell and Retinoic Acid Metabolism Marker (RQ5510)

ALDH1A1 Antibody IF. Immunofluorescence analysis of FFPE human A549 cells stained with ALDH1A1 Antibody / Cancer Stem Cell and Retinoic Acid Metabolism Marker (green). Aldehyde dehydrogenase 1 family member A1 / ALDH1A1 shows predominantly cytoplasmic fluorescence within tumor epithelial cells, consistent with the known localization of this aldehyde detoxification and retinoic acid biosynthesis enzyme. Nuclei are counterstained with DAPI (blue). HIER: boil tissue sections in 10 mM citrate buffer, pH6, for 20 min followed by cooling before testing.

ALDH1A1 Antibody IF. Immunofluorescence analysis of FFPE human A431 cells stained with ALDH1A1 Antibody / Cancer Stem Cell and Retinoic Acid Metabolism Marker (green). Cytoplasmic ALDH1A1 fluorescence is detected throughout the epithelial tumor cell population, consistent with the established localization of Aldehyde dehydrogenase 1 family member A1 / ALDH1A1 in metabolically active carcinoma cells. Nuclear counterstaining was performed with DAPI (blue). HIER: boil tissue sections in 10 mM citrate buffer, pH6, for 20 min followed by cooling before testing.

ALDH1A1 Antibody FACS. Flow cytometry analysis of human HepG2 cells using ALDH1A1 Antibody / Cancer Stem Cell and Retinoic Acid Metabolism Marker. The ALDH1A1-stained population (blue) demonstrates a clear rightward fluorescence shift relative to the isotype control (green) and unstained cells (red), consistent with expression of Aldehyde dehydrogenase 1 family member A1 / ALDH1A1 in hepatocellular carcinoma-derived cells. Cells were blocked with goat sera prior to staining and tested at 1 ug antibody per million cells.

ALDH1A1 Antibody FACS. Flow cytometry analysis of human HL60 cells stained with ALDH1A1 antibody. The ALDH1A1-positive population (blue) shows a distinct fluorescence shift compared to the isotype control (green) and unstained cells (red), supporting expression of Aldehyde dehydrogenase 1 family member A1 / ALDH1A1 in this promyelocytic leukemia cell line. Cells were blocked with goat sera prior to staining and tested at 1 ug antibody per million cells.

ALDH1A1 Antibody Breast Cancer IHC. Immunohistochemistry analysis of FFPE human breast carcinoma stained with ALDH1A1 Antibody / Cancer Stem Cell and Retinoic Acid Metabolism Marker. Tumor epithelial cells demonstrate moderate to strong cytoplasmic HRP-DAB brown staining for Aldehyde dehydrogenase 1 family member A1 / ALDH1A1, with heterogeneous expression across malignant cell clusters consistent with the known association of ALDH1A1 with stem-like tumor cell populations and metabolic adaptation in breast cancer. Hematoxylin counterstain highlights nuclei in blue. HIER: boil tissue sections in 10 mM citrate buffer, pH6, for 20 min followed by cooling before testing.

ALDH1A1 Antibody Renal Cancer IHC. Immunohistochemistry analysis of FFPE human renal carcinoma stained with ALDH1A1 Antibody / Cancer Stem Cell and Retinoic Acid Metabolism Marker. Tumor epithelial cells display cytoplasmic and partial membranous HRP-DAB brown staining for Aldehyde dehydrogenase 1 family member A1 / ALDH1A1, consistent with expression of this aldehyde detoxification and retinoic acid metabolism enzyme in renal tumor tissue. Hematoxylin counterstain highlights nuclei in blue. HIER: boil tissue sections in 10 mM citrate buffer, pH6, for 20 min followed by cooling before testing.

ALDH1A1 Antibody Mouse Stomach IHC. Immunohistochemistry analysis of FFPE mouse stomach tissue stained with ALDH1A1 Antibody / Cancer Stem Cell and Retinoic Acid Metabolism Marker. Strong cytoplasmic HRP-DAB brown staining is observed within the gastric epithelial lining, consistent with expression of Aldehyde dehydrogenase 1 family member A1 / ALDH1A1 in metabolically active epithelial cells involved in aldehyde detoxification and retinoic acid metabolism. Hematoxylin counterstain highlights nuclei in blue. HIER: boil tissue sections in 10 mM citrate buffer, pH6, for 20 min followed by cooling before testing.

ALDH1A1 Antibody Rat Stomach IHC. Immunohistochemistry analysis of FFPE rat stomach tissue stained with ALDH1A1 Antibody / Cancer Stem Cell and Retinoic Acid Metabolism Marker. Gastric epithelial cells exhibit cytoplasmic HRP-DAB brown staining for Aldehyde dehydrogenase 1 family member A1 / ALDH1A1, consistent with the known role of this aldehyde-metabolizing enzyme in gastrointestinal epithelial metabolism and cellular detoxification pathways. Hematoxylin counterstain highlights nuclei in blue. HIER: boil tissue sections in 10 mM citrate buffer, pH6, for 20 min followed by cooling before testing.

Western blot testing of human 1) placenta, 2) A549 and 3) HepG2 lysate with ALDH1A1 antibody. Expected molecular weight ~55 kDa.

ALDH1A1 Antibody Liver Lung Kidney WB. Western blot testing of 1) rat liver, 2) rat lung, 3) rat kidney, 4) mouse liver, 5) mouse lung and 6) mouse kidney lysate with ALDH1A1 antibody. Expected molecular weight ~55 kDa.