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Home >> Antibodies >> Alcohol dehydrogenase 4 Antibody / ADH4

Alcohol dehydrogenase 4 Antibody / ADH4 (F54534)

  Catalog No Formulation Size Price (USD)  
Image F54534-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 369
Image
F54534-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 149
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Alcohol dehydrogenase 4 antibody. Predicted molecular weight ~40 kDa.
Western blot testing of mouse heart tissue with Alcohol dehydrogenase 4 antibody. Predicted molecular weight ~40 kDa.
IHC testing of FFPE human colon carcinoma tissue with Alcohol dehydrogenase 4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HEK293 cells with Alcohol dehydrogenase 4 antibody; Blue=isotype control, Green= Alcohol dehydrogenase 4 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt P08319
Localization Cytoplasmic
Applications Flow cytometry : 1:25 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:25
Western blot : 1:500-1:2000
Limitations This Alcohol dehydrogenase 4 antibody is available for research use only.
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Description

This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Alcohol dehydrogenase 4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 319-348 from the human protein was used as the immunogen for the Alcohol dehydrogenase 4 antibody.

Storage

Aliquot the Alcohol dehydrogenase 4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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