Albumin Antibody for IF / Cytoplasmic Serum Protein Localization Antibody [clone ALB/2356] (V7528)

Albumin Antibody HepG2 IF. Immunofluorescence analysis of paraformaldehyde-fixed human HepG2 cells using Albumin antibody (green) shows cytoplasmic staining with a punctate, perinuclear distribution consistent with localization within the secretory pathway, including endoplasmic reticulum and Golgi-associated compartments, while nuclei are counterstained with Reddot (red); detection was performed with clone ALB/2356.

Western blot testing of human HepG2 cell lysate with Albumin antibody (clone ALB/2356). Predicted molecular weigth ~66 kDa.

Albumin Antibody HepG2 FACS. Flow cytometry analysis of paraformaldehyde-fixed human HepG2 cells using Albumin antibody shows a clear rightward shift in fluorescence intensity compared to the isotype control, consistent with intracellular detection of ALB and supporting cytoplasmic localization of this liver-derived serum protein; blue histogram represents Albumin antibody staining and red histogram represents isotype control, with detection performed using clone ALB/2356.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Albumin antibody (clone ALB/2356). These results demonstrate the foremost specificity of the ALB/2356 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Albumin antibody (clone ALB/2356) as confirmation of integrity and purity.

Albumin Antibody Liver Species Comparison WB. Western blot analysis of human, mouse, rat, hamster, and guinea pig liver tissue lysates using Albumin antibody detects a prominent band at approximately 65-70 kDa in human liver, consistent with the predicted molecular weight of Albumin (ALB), with minimal signal observed in other species under these conditions; detection was performed with clone ALB/2356.