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Home >> Antibodies >> ALAS2 Antibody / ASB / 5-aminolevulinic acid synthase 2

ALAS2 Antibody / ASB / 5-aminolevulinic acid synthase 2 (RQ6930)

  Catalog No Formulation Size Price (USD)  
Image RQ6930 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 419
Immunofluorescent staining of FFPE human PC-3 cells with ALAS2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human 293T, 2) rat liver, 3) rat heart, 4) mouse liver and 5) mouse heart lysate with ALAS2 antibody. Predicted molecular weight ~65 kDa.
Flow cytometry testing of human K562 cells with ALAS2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ALAS2 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P22557
Applications Western blot : 0.5-1 ug/ml
Flow cytometry : 1-3ug/million cells
Immunofluorescence : 5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This ALAS2 antibody is available for research use only.
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Description

Delta-aminolevulinate synthase 2 also known as ALAS2 is a protein that in humans is encoded by the ALAS2 gene. The product of this gene specifies an erythroid-specific mitochondrially located enzyme. The encoded protein catalyzes the first step in the heme biosynthetic pathway. Defects in this gene cause X-linked pyridoxine-responsive sideroblastic anemia. Alternatively spliced transcript variants encoding different isoforms have been identified.

Application Notes

Optimal dilution of the ALAS2 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids M1-D190) was used as the immunogen for the ALAS2 antibody.

Storage

After reconstitution, the ALAS2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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